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Journal of Plant Diseases and Protection (2009) 116, 2-6

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M.K. Singh, V. Chandel, V. Hallan, R. Ram and A.A. Zaidi (2009)
Occurrence of Peanut stripe virus on patchouli and raising of virus-free patchouli plants by meristem tip culture
Journal of Plant Diseases and Protection 116 (1), 2-6
Abstract: Patchouli (Pogostemon cablin) was found infected with a viral disease showing mosaic symptoms on the leaves in the Chandpur farm at Institute of Himalayan Bioresource Technology campus, Palampur, Himachal Pradesh, India. After ELISA, RT-PCR, IC-PCR and sequencing, the virus was identified as Peanut stripe virus (PStV). Sequence comparison with corresponding sequences of established potyviruses showed 52-93% homology at nucleotide level. Peanut stripe virus was eliminated from Patchouli plants using meristem tip culture. MS medium amended with benzylaminopurine (2 mg l) and indole3-butyric acid (0.05 mg l) was used for shoot proliferation and rooting of plants, respectively. More than 88.89% virus-free plants were obtained from 0.2 mm of meristem as shown by indirect ELISA whereas 80.55% virus-free plants were obtained from 0.3 mm size of meristem as indicated by RT-PCR.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Aijaz Asghar Zaidi, Raja Ram, Vanita Chandel

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
control - general


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Peanut stripe virus Patchouli (Pogostemon) India