Journal of Phytopathology - Phytopathologische Zeitschrift (2002) 150, 564-568

Z.W. Subr, U. Kastirr and T. Kühne (2002)
Subtractive cloning of DNA from Polymyxa graminis- an obligate parasitic plasmodiophorid
Journal of Phytopathology - Phytopathologische Zeitschrift 150 (10), 564-568
Abstract: A simple subtractive hybridization was applied for cloning of Polymyxa graminis deoxyribonucleic acid (DNA). Total DNA preparations from concentrated P. graminis resting spores and from roots of non-infested (healthy) barley were digested with different restriction enzymes and batch hybridized, followed by cloning in a plasmid vector. Sequencing and blast analysis of coincidentally selected clones enabled construction of polymerase chain reaction primers specific to P. graminis DNA. Four Polymyxa-specific primers showed different affinities to DNA of type I and type II P. graminis and to DNA of P. betae.
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Link to article at publishers website
Database assignments for author(s): Ute Kastirr

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Polymyxa graminis