Journal of Phytopathology - Phytopathologische Zeitschrift (1996) 144, 363-369

P.W. Tooley, M.M. Carras and K.F. Falkenstein (1996)
Relationships among group IV Phytophthora species inferred by restriction analysis of the ITS2 region
Journal of Phytopathology - Phytopathologische Zeitschrift 144 (7-8), 363-369
Abstract: The polymerase chain reaction (PCR) was used to amplify the ITS2 region of nuclear ribosomal DNA from six Phytophthora species which comprise taxonomic Group IV. Digestion of the ca. 600 by PCR product with restriction enzymes AluI, Dral, Hhal, Hinf l, Mspl, and Taql revealed variation which allowed relationships among the species to be assessed. P. infestans, P. mirabilis and P. phaseoli were indistinguishable from one another with all enzymes tested. With AluI and TaqI, P. ilicis, P. colocasiae, and P. hibernalis each showed unique banding patterns different from the common banding pattern shared by P. infestans, P. mirabilis, and P. hibernalis. Dral allowed differentiation of P. ilicis and P. colocasiae from P. infestans, P. mirabilis, P. phaseoli, and P. hibernalis, all of which shared a common banding pattern. Hhal allowed differentiation of P. colocasiae and P. hibernalis from P. infestans, P. mirabilis, P. phaseoli, and P. ilicis. Hinf I allowed differentiation of P. ilicis and P. hibernalis (each of which showed a unique banding pattern) from P. infestans, P. mirabilis, P. phaseoli, and P. colocasiae. Mspl allowed differentiation of P. hibernalis from the other five species. Species groupings determined by restriction analysis of ITS2 were consistent with those based on morphological criteria. These results show that restriction analysis of PCR-amplified TS2 regions can be useful as an adjunct to morphological criteria in Phytophthora species identification.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytophthora infestans
	Phytophthora colocasiae
	Phytophthora phaseoli
	Phytophthora hibernalis
	Phytophthora mirabilis