Journal of Phytopathology - Phytopathologische Zeitschrift (1996) 144, 29-32

C. Huguenot, M.T. Furneaux, J. Clare and R.I. Hamilton (1996)
Serodiagnosis of Pepper veinal mottle virus in West Africa using specific monoclonal antibodies in DAS-ELISA
Journal of Phytopathology - Phytopathologische Zeitschrift 144 (1), 29-32
Abstract: In order to develop a reliable diagnostic test for pepper veinal mottle virus (PVMV), a panel of monoclonal antibodies (mAbs), raised to a Nigerian isolate (PVMW-Ni), was evaluated by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) on infected pepper samples from seven West African countries. PVMV from different geographical locations was serologically very homogeneous and no significant epitope differences were detected even with a very sensitive double-mAb sandwich ELISA. This ELISA format was optimized with mAb 6C12 and its application in a diagnostic test resulted in the identification of PVMV in Burkina Faso, Togo and Sénégal where it had not been previously reported.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pepper veinal mottle virus		Burkina Faso
	Pepper veinal mottle virus		Ethiopia
	Pepper veinal mottle virus		Kenya
	Pepper veinal mottle virus		Nigeria
	Pepper veinal mottle virus		Togo
	Pepper veinal mottle virus		South Africa
	Pepper veinal mottle virus	Green pepper/chilli (Capsicum)	Senegal