Journal of Phytopathology - Phytopathologische Zeitschrift (1996) 144, 257-263
M. Bashir and R.O. Hampton (1996)
Serological and biological comparisons of blackeye cowpea mosaic and cowpea aphid-borne mosaic potyvirus isolates seed-borne in Vigna unguiculata (L.) Walp. germplasm
Journal of Phytopathology - Phytopathologische Zeitschrift 144 (5), 257-263
Abstract: Serological and biological comparisons were made among 45 seed-borne isolates of blackeye cowpea mosaic (BICMV) and 54 seed-borne isolates of cowpea aphid-borne mosaic (CAbMV) potyviruses derived from Cowpea seedlots or young nursery-grown seedlings comprising 2112 germplasm accessions or pre-introduction seedlots of Vigna unguiculata. Isolates were identified by DAS-ELISA using polyclonal immunoglobulin G specific for these viruses. Twenty isolates of BICMV and 32 isolates of CAbMV were also compared by ACP-ELISA with selected monoclonal antibodies and by SDS-immunodiffusion. By all approaches, isolates of BlCMV were clearly distinguished from CAbMV isolates. Isolate comparisons on selected Cowpea genotypes (TVu-401, TVu-1582, TVu-2657, and TVu-3433) partitioned most isolates into two distinct groups. A few isolates seed-borne in Indian cv. Pusa Phalguni, however, were clearly BICMV by all serological tests, but behaved as CAbMV in definitive Cowpea genotypes. Although BlCMV is generally considered to be a 'new world virus', both BICMV and CAbMV occurred in V. unguiculata seedlots originating in 'old-world regions', including Afghanistan, Botswana, Nigeria, Senegal, and South Africa. Seed-borne CAbMV was isolated from 6 of 155 tested US V. unguiculata Germplasm accessions originating, respectively, in Afghanistan (2), Botswana (2), India (1), and Pakistan (1).
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
