Journal of Phytopathology - Phytopathologische Zeitschrift (1995) 143, 683-687

Ki Hyun and Won Mok Park (1995)
Complementary DNA hybridization analysis of double-stranded RNAs associated with Cucumber mosaic virus-As strain
Journal of Phytopathology - Phytopathologische Zeitschrift 143 (11-12), 683-687
Abstract: Virus-specific double-stranded (ds) RNAs were isolated from leaves of Nicotiana tabacum cv. Xanthinc infected with the As or Y strains of cucumber mosaic virus (CMV). These dsRNAs on agarose gels produced 5 bands corresponding, respectively to three genomic RNAs (dsRNA1, −2, −3), the subgenomic mRNA for coat protein (dsRNA4). and satellite dsRNA (dsRNA5) with estimated molecular weights of respectively, 2.5, 2.4, 1.45, 0.69 and 0.28 × 10³ kD. In northern-blot analysis, a 1 kbp complementary DNA (cDNA) to CMV-As RNA4 hybridized strongly with dsRNA3 and dsRNA4 extracted from CMV-As infected tobacco, but did not hybridize with dsRNA1, dsRNA2, or satellite dsRNA (dsRNA5). In slot-blot analysis, the cDNA hybridized strongly with virus-specific dsRNAs isolated from CMV infected plants, but not with dsRNAs of TMV-Korean common, potato virus Y-VN, or total RNA of healthy tobacco plants.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus