Journal of Phytopathology - Phytopathologische Zeitschrift (1995) 143, 643-646

Ki Hyun Ryu, K.E. Yoon and Won Mok Park (1995)
Detection by RT-PCR of Cymbidium mosaic virus in orchids
Journal of Phytopathology - Phytopathologische Zeitschrift 143 (11-12), 643-646
Abstract: The cymbidium mosaic potexvirus (CyMV) coat-protein gene was selected for the design of oligonucleotide primers. Reverse transcription-polymerase chain reaction (RT-PCR) was performed with 20-mer CyMV coat-protein-specific primers to amplify a 692 base-pair fragment from crude nucleic acid extracts of virus-infected orchid-leaf tissue. The lowest concentration of template viral RNA required to detect the virus was 10 fg. No PCR products were obtained when odontoglossum ringspot virus (ORSV) RNA or extracts of healthy Cymbidium sp. were used as templates in RT-PCR using the same primers. A 2μl aliquot of crude nucleic-acid extracts from 0.1 g of the CyMV-infected leaf tissue was sufficient to yield a diagnostic PCR product.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ki Hyun Ryu

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cymbidium mosaic virus	Cymbidium (crop)