Journal of Phytopathology (2016) 164, 904-912

Jing Jin, Jianguo Shen, Wei Cai, Furong Liao, Fangluan Gao, Xihong Chen and Zujian Wu (2016)
A cocktail ELISA semi-nested RT-PCR assay to detect Bean pod mottle virus in soya bean seeds
Journal of Phytopathology 164 (11-12), 904-912
Abstract: Bean pod mottle virus (BPMV) has been identified as an important pathogen for plant quarantine in China because large quantities of soya bean seeds (approximately 7 × 10⁷ tons) are imported annually. To develop a practical detection programme for BPMV, a cocktail enzyme-linked immunosorbent assay (ELISA) nested RT-PCR using a combination of serological and molecular methods was designed for soya bean seeds. The single-vessel detection assay was performed in a 96-well ELISA plate, which served as a carrier for the subsequent nested RT-PCR assay. Assay specificity was demonstrated by the production of the expected 330- and 296-bp bands using the external and internal primers, respectively. This method was 10⁴-fold more sensitive than immunocapture-RT-PCR (IC-RT-PCR). In particular, it is important to note that this assay resulted in successful micro-extraction from soya bean seeds and combined the advantages of each individual technique. The cocktail ELISA nested RT-PCR is a specific, sensitive, rapid and economical procedure to rapidly identify and characterize BPMV and could be suitable for both primary-level platforms and laboratories.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Zu-Jian Wu

Research topic(s) for pests/diseases/weeds:
quarantine treatments/regulations/aspects
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Comovirus siliquae	Soybean (Glycine max)