Journal of Phytopathology (2011) 159, 130-132

Helena Plchova, Tomas Moravec, Petr Dedic and Noemi Cerovska (2011)
Expression of recombinant Potato leafroll virus structural and non-structural proteins for antibody production
Journal of Phytopathology 159 (2), 130-132
Abstract: Vector pMPM-A4-Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp_43-616 and RdRp_304-537 were chosen for expression. A high level of CP and RdRp_304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
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Link to article at publishers website
Database assignments for author(s): Noemi Cerovska

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato leafroll virus