Journal of Phytopathology (2010) 158, 609-615

Takahiro Asano, Masako Senda, Haruhisa Suga and Koji Kageyama (2010)
Development of multiplex PCR to detect five Pythium species related to turfgrass diseases
Journal of Phytopathology 158 (9), 609-615
Abstract: The objective of this study was to develop multiplex PCR detection method for five Pythium species associated with turfgrass diseases, Pythium aphanidermatum, Pythium arrhenomanes, Pythium graminicola, Pythium torulosum and Pythium vanterpoolii. Species-specific primers and two common primers were designed based on the sequences of the internal transcribed spacer region of ribosomal DNA. Another primer set by which all organisms would be amplified in 18S rDNA was used as a positive control. When these total nine primers were applied to the multiplex PCR, all species were individually discriminated in the mixture of five species culture DNA. Furthermore, all five Pythium species were detected in naturally infected plants using the multiplex PCR.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Koji Kageyama

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pythium aphanidermatum	Grasses/turf/rangeland
	Pythium arrhenomanes	Grasses/turf/rangeland
	Pythium torulosum	Grasses/turf/rangeland
	Pythium vanterpoolii	Grasses/turf/rangeland
	Pythium graminicola	Grasses/turf/rangeland