Journal of Phytopathology (2010) 158, 382-386

Paolo Margaria, Sabrina Palmano and Massimo Turina (2010)
Cloning of the glyceraldehyde 3-phosphate dehydrogenase gene of flavescence dorée phytoplasma and development of serological and molecular tools for studying its expression
Journal of Phytopathology 158 (5), 382-386
Abstract: The glyceraldehyde 3-phosphate dehydrogenase (gapA) gene codes for a protein involved in the glycolytic pathway and is commonly used in Real-Time RT-PCR quantification studies as housekeeping gene. In this work we cloned and sequenced the full-length gapA gene from Flavescence dorée phytoplasma (FDp). A ~35 kDa recombinant GapA protein was over-expressed in Escherichia coli, purified and used as antigen to raise anti-GapA rabbit polyclonal antibodies. The antiserum detected the GapA protein by western blot analysis of total protein extracts of FDp-infected experimental host (Catharanthus roseus) and grapevine plants collected in the field. We also developed an FDp-specific gapA Taqman Real-Time RT-PCR assay suitable for quantification overtime of gapA mRNA in infected plants.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Massimo Turina, Paolo Margaria, Sabrina Palmano

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma vitis	Grapevine (Vitis)