Journal of Phytopathology (2005) 153, 68-72

V. Ortiz, S. Castro and J. Romero (2005)
Optimization of RT-PCR for the detection of Bean leaf roll virus in plant hosts and insect vectors
Journal of Phytopathology 153 (2), 68-72
Abstract: The detection of luteoviruses by reverse transcription polymerase chain reaction (RT-PCR) depends on the adequate quality and quantity of extracted viral nucleic acids. We have optimized the detection of Bean leaf roll virus (BLRV) using selective precipitation by LiCl of viral RNA from a small quantity of infected plant tissues and insect vectors. The optimal template for PCR was 15 µl of RT reaction mixture. BLRV was detected in different plant hosts and aphid vectors and Aphis fabae, previously considered to be a non-vector of BLRV, was found to acquire the virus from infected plants.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Javier Romero

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Luteovirus phaseoli