Journal of Pest Science (2011) 84, 457-469

Hari Bahadur Khatri-Chhetri, Govinda Prasad Timsina, Hira Kaji Manandhar and Maurice Moens (2011)
Potential of Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae)
Journal of Pest Science 84 (4), 457-469
Abstract: Heterorhabditis indica CK2 and H. indica CK6) were used in a series of bioassays against the insect. All isolates showed an increased dispersal in response to H. longipennis. Nematodes were more attracted towards third instar larvae than to second instars. Differences in penetration and multiplication in the insect were observed amongst the seven isolates. Steinernema lamjungense LMT5, S. everestense DKP4 and S. abbasi CS1 caused greater mortality than other isolates to different developmental stages. Pupae and second instar larvae were more susceptible than third instar larvae. Significant differences were observed in LT₅₀ values of the isolates against different stages of H. longipennis. Three isolates (S. lamjungense LMT5, S. everestense DKP4 and S. abbasi CS1) along with a commonly used insecticide (chlorpyrifos) were tested against this insect in pot and field experiments. In pot experiments using maize and cabbage as a host crop, S. lamjungense LMT5 and S. everestense DKP4 performed better than S. abbasi CS1 and yielded a mortality comparable with chlorpyrifos. Similar results were observed in field experiments 3 weeks after nematode application. These experiments overall suggest S. lamjungense LMT5 to be a promising biocontrol agent against H. longipennis followed by S. everestense DKP4 and S. abbasi CS1.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Holotrichia longipennis	Cabbage (Brassica oleracea)	Nepal
	Holotrichia longipennis	Maize/corn (Zea mays)	Nepal
Steinernema lamjungense (entomopathogen)	Holotrichia longipennis		Nepal
Steinernema everestense (entomopathogen)	Holotrichia longipennis		Nepal