Journal of Medical Entomology (2016) 53, 1410-1414

Gustavo Ponce-García, Samantha Del Río-Galvan, Roberto Barrera, Karla Saavedra-Rodriguez, Karina Villanueva-Segura, Gilberto Felix, Manuel Amador and Adriana E. Flores (2016)
Knockdown resistance mutations in Aedes aegypti (Diptera: Culicidae) from Puerto Rico
Journal of Medical Entomology 53 (6), 1410-1414
Abstract: Permethrin resistance is widespread in Aedes aegypti (L.), the main dengue, zika, and chikungunya virus vector in Latin America and the Caribbean. A common mechanism of resistance to pyrethroids—knockdown resistance (kdr)—is conferred through mutations in the insect's voltage-dependent sodium channel. In this mosquito, around 10 replacement substitutions in the voltage-gated sodium channel gene (vgsc) have been reported in pyrethroid-resistant strains. Two of these mutations, named Ile1,016 and Cys1,534, are widespread in mosquito populations from Latin America and the Caribbean. This study assessed the levels of permethrin resistance and the frequency of two kdr mutations in eight Ae. aegypti populations collected in Puerto Rico in 2013. Permethrin resistance factors ranged from 33–214-fold relative to the New Orleans reference strain. The frequency of kdr mutation Ile1,016 ranged from 0.65 to fixation (1.0), and for Cys1,534 frequencies varied from 0.8 to fixation. Alarmingly, two populations—Carolina and Caguas—reached fixation at both loci. Our results suggest that permethrin effectiveness for Ae. aegypti control is compromised in these collections from Puerto Rico.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Roberto Barrera, Adriana E. Flores

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aedes aegypti		Puerto Rico