Journal of Medical Entomology (2007) 44, 376-380

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Nigel W. Beebe, Peter I. Whelan, Andrew F. van den Hurk, Scott A. Ritchie, Sarah Corcoran and Robert D. Cooper (2007)
A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region
Journal of Medical Entomology 44 (2), 376-380
Abstract: Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedes aegypti (L.) and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic and exotic container mosquito species resulted in the development of a polymerase chain reaction diagnostic procedure that uses a restriction enzyme to cut the internal transcribed spacer region 1 of the ribosomal DNA to separate Ae. aegypti and Ae. albopictus from a number of common local container mosquito species which can be used at any stage of the life cycle, including eggs up to 8 wk of age. Identification was possible using desiccated or alcohol-preserved specimens with a response time of <24 h after receipt of the specimens.
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Database assignments for author(s): Nigel W. Beebe, Scott A. Ritchie

Research topic(s) for pests/diseases/weeds:

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Aedes notoscriptus Australia (NT+QLD)
Aedes albopictus Papua New Guinea
Aedes albopictus Thailand
Aedes albopictus Vietnam
Aedes albopictus Australia (NT+QLD)
Aedes albopictus East Timor
Aedes aegypti Myanmar
Aedes aegypti Papua New Guinea
Aedes aegypti Thailand
Aedes aegypti Vietnam
Aedes aegypti Vanuatu
Aedes aegypti Australia (NT+QLD)
Aedes aegypti East Timor
Aedes tremulus Australia (NT+QLD)
Aedes scutellaris Papua New Guinea
Aedes scutellaris Australia (NT+QLD)