Journal of Medical Entomology (2006) 43, 707-712

Kevin B. Temeyer, John H. Pruett, Pia M. Untalan and Andrew C. Chen (2006)
Baculovirus expression of BmAChE3, a cDNA encoding an acetylcholinesterase of Boophilus microplus (Acari: Ixodidae)
Journal of Medical Entomology 43 (4), 707-712
Abstract: The complete cDNA sequence encoding a Boophilus microplus (Canestrini) (Acari: Ixodidae) acetylcholinesterase (AChE3) was expressed in the baculovirus system. The recombinant AChE3 protein (rBmAChE3) was secreted as a soluble form into the cell culture medium and was identified as a functional AChE by substrate specificity and by inhibition with the AChE-specific inhibitors eserine sulfate and BW284c51. Inhibition kinetics of rBmAChE3, in the presence of the organophosphate paraoxon, revealed sensitivity comparable with that of adult, organophosphate-susceptible neural AChE. To our knowledge, this is the first report of the cloning and successful expression of a functional ixodid AChE.
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Database assignments for author(s): Kevin B. Temeyer, John H. Pruett, Andrew C. Chen

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Rhipicephalus microplus