Journal of Insect Science (2014) 14 (171), 1-6

Petr Stary, Nickolas G. Kavallieratos, Andjeljko Petrovic, Vladimir Zikic, Ehsan Rakhshani, Snezana Tomanovic, Zeljko Tomanovic and Jan Havelka (2014)
Interference of field evidence, morphology, and DNA analyses of three related Lysiphlebus aphid parasitoids (Hymenoptera: Braconidae: Aphidiinae)
Journal of Insect Science 14 (171), 1-6
Abstract: This study provides evidence on integrating the morphological, field, and laboratory data, and application of the cytochrome oxidase subunit I (COI) barcoding gene to the three asexual or sexual Lysiphlebus spp., i.e., Lysiphlebus cardui (Marshall), Lysiphlebus confusus Tremblay and Eady and Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae: Aphidiinae). New aphid– invasive plant association, Aphis fabae Scopoli (Hemipreta: Aphididae) on Impatiens glandulifera Royle, has been used in the same model area in the Czech Republic under the same sampling and rearing method for several consecutive years and throughout the season. For molecular identification of these three species, we used DNA sequences of the barcoding region of the mitochondrial COI gene. Although our results confirmed ecological and morphological differences among L. cardui, L. confusus, and L. fabarum, genetic analysis on the basis of COI mitochondrial barcoding gene does not support species status of the mentioned Lysiphlebus taxa. The level of morphological differentiation in these Lysiphlebus Förster species is in accordance with the usual species variability within subfamily Aphidiinae. However, it should be examined how appearance of asexual lineages affects the morphological or genetical variability.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Petr Stary, Zeljko Tomanovic, Nickolas G. Kavallieratos, Ehsan Rakhshani, Snezana Tomanovic, Jan Havelka

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
surveys/distribution/isolation
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis fabae		Czech Republic
	Aphis farinosa		Czech Republic
Lysiphlebus fabarum (parasitoid)	Aphis fabae		Czech Republic
Lysiphlebus cardui (parasitoid)	Aphis fabae		Czech Republic
Lysiphlebus confusus (parasitoid)	Aphis fabae		Czech Republic
Lysiphlebus confusus (parasitoid)	Aphis farinosa		Czech Republic