Journal of Insect Science (2013) 13 (56), 1-13

Jiao Zhou, Haolin Zhang, Juan Li, Xia Sheng, Shixiang Zong, Youqing Luo, Kentaro Nagaoka, Qiang Weng, Gen Watanabe and Kazuyoshi Taya (2013)
Molecular cloning and expression profile of a Halloween gene encoding CYP307A1 from the seabuckthorn carpenterworm, Holcocerus hippophaecolus
Journal of Insect Science 13 (56), 1-13
Abstract: 20-Hydroxyecdyone, an active form of ecdysteroid, is the key hormone in insect growth and development. Halloween genes encode ecdysteroidogenic enzymes, including cytochrome P450 monooxygenase. CYP307A1 (spook) is accepted as an enzyme acting in the so-called 'black box' that includes a series of hypothetical and unproven reactions that finally result in the oxidation of 7-dehydrocholesterol to diketol. In this study, the Holcocerus hippophaecolus Hua (Lepidoptera: Cossidae) CYP307A1 (HhSpo) gene was identified and characterized. The obtained cDNA sequence was 2084 base pairs with an open reading frame of 537 animo acids, in which existed conserved motifs of CYP450 enzymes. The transcript profiles of HhSpo were analyzed in various tissues of final instar larvae. The highest expression was observed in the prothoracic gland, while expression level was low but significant in other tissues. These results suggest that the sequence character and expression profile of HhSpo were well conserved and provided the basic information for its functional analysis.
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Link to article at publishers website
Database assignments for author(s): Shixiang Zong

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Eogystia hippophaecolus	Sea buckthorn (Hippophaë rhamnoides)	China (NE)