Journal of Insect Science (2006) 6 (46), 2-3

Taofic Alabi, Frederic Francis, Edwin De Paw and Eric Haubruge (2006)
Proteomics to identify odorant binding proteins (OBPs) and chemosensory proteins (CSPs) from the antennae and tarsi of Tribolium brevicornis
Journal of Insect Science 6 (46), 2-3
in M. Adams et al. [editors] Abstracts of the Fifth International Symposium on Molecular Insect Science (May 20-24, 2006, Tucson, Arizona USA), 76 pp.
Abstract: Chemoreception in insects is mediated by small soluble proteins that are abundantly present in the aqueous lymph of chemosensilla and that interact with odorant molecules and pheromones on their way to and from olfactory receptor. Two major classes of such proteins have been described: odorant binding proteins (OBPs) and chemosensory proteins (CSPs). A proteomic approach based on two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), in which proteins are separated according to charge (pI) by isoelectric focusing (IEF) and according to size (Mr) by SDS-PAGE, was performed for the resolution of complex mixtures of proteins from antennae and Tarsi of Tribolium brevicornis. The proteins were then silver-stained and analysed by Matrix assisted laser desorption time of flight MS (MALDI-TOF) or by Electrospray (ESI) coupled with tandem Mass Spectrometry (MS-MS). Proteins from this Tribolium species was found to present sequence similarities to OBPs and CSPs recently discovery in several other insect orders. Development of proteomic studies was discussed in term of efficiency in functional and evolutional entomology.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Frédéric Francis

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tribolium brevicornis