Journal of General Virology (2013) 94, 1421-1425

Yuanyuan Ma, Wei Wu, Hongyan Chen, Qifei Liu, Dongsheng Jia, Qianzhuo Mao, Qian Chen, Zujian Wu and Taiyun Wei (2013)
An insect cell line derived from the small brown planthopper supports replication of Rice stripe virus, a tenuivirus
Journal of General Virology 94 (6), 1421-1425
Abstract: A cell line from the small brown planthopper (SBPH; Laodelphax striatellus) was established to study replication of rice stripe virus (RSV), a tenuivirus. The SBPH cell line, which had been subcultured through 30 passages, formed monolayers of epithelial-like cells. Inoculation of cultured vector cells with RSV resulted in a persistent infection. During viral infection in the SBPH cell line, the viral non-structural protein NS3 co-localized with the filamentous ribonucleoprotein particles of RSV, as revealed by electron and confocal microscopy. The knockdown of NS3 expression due to RNA interference induced by synthesized double-stranded RNAs from the NS3 gene significantly inhibited viral infection in the SBPH cell line. These results demonstrated that NS3 of RSV might be involved in viral replication or assembly. The persistent infection of the SBPH cell line by RSV will enable a better understanding of the complex relationship between RSV and its insect vector.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Zu-Jian Wu

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases
rearing/culturing/mass production

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Laodelphax striatellus
	Tenuivirus oryzaclavatae