Journal of General Virology (2005) 86, 2107-2113

Marcel Prins, Dick Lohuis, Arjen Schots and Rob Goldbach (2005)
Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus
Journal of General Virology 86 (7), 2107-2113
Abstract: Rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. Here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus Tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. High cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were established. Of these antibodies, two led to high levels of resistance against this plant virus. Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field.
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Database assignments for author(s): Marcel Prins

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Orthotospovirus tomatomaculae