Journal of General Virology (2004) 85, 1739-1744

S.D. Zvereva, P.A. Ivanov, M.V. Skulachev, A.G. Klyushin, Yu. L. Dorokhov and J.G. Atabekov (2004)
Evidence for contribution of an internal ribosome entry site to intercellular transport of a tobamovirus
Journal of General Virology 85 (6), 1739-1744
Abstract: Previously, it has been shown that tobacco mosaic virus (TMV) U1 and crucifer-infecting TMV contain a 75 nt internal ribosome entry site (IRES) upstream of movement protein (MP) gene (IRES^U1_MP,75 and IRES^CR_MP,75, respectively). A movement-deficient TMV mutant, KK6, has been constructed previously [Lehto, K., Grantham, G. L. and Dawson, W. O. (1990). Virology 174, 145-157] by insertion of the second coat protein subgenomic promoter (CP SGP-2) upstream of the MP gene, in addition to the natural CP SGP-1. Here, the authors compare the efficiency of movement function expression by KK6 and a derivative, K86, obtained by insertion of IRES^CR_MP,75 between the CP SGP-2 and MP genes resulting in restoration of IRES^CR_MP,75 function in the 5'-untranslated sequence of the I₂ subgenomic RNA of K86. The data indicate that the efficiency of K86 movement was largely restored by this insertion, which was apparently due to the translation-enhancing ability of IRES^CR_MP,75.
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Database assignments for author(s): Joseph G. Atabekov

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tobacco mosaic virus