Journal of General Virology (2001) 82, 1529-1532

Ewa Sadowy, Anna Maasen, Marek Juszczuk, Chantal David, Wlodzimierz Zagórski-Ostoja, Bruno Gronenborn and M. Danuta Hulanicka (2001)
The ORF0 product of Potato leafroll virus is indispensable for virus accumulation
Journal of General Virology 82 (6), 1529-1532
Abstract: Using a cDNA expression cassette in combination with agroinoculation of potato leaf discs we have investigated the role the protein encoded by ORF0 of Potato leafroll virus (PLRV) and have shown its importance for virus accumulation. Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells. They did not, however, affect translation of ORF1 and ORF2. We therefore conclude that ORF0 of PLRV produces a protein essential for virus accumulation, a hitherto undescribed finding.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato leafroll virus