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Journal of General Virology (2001) 82, 1503-1508

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O. I. Kiselyova, I. V. Yaminsky, E. M. Karger, O. Yu Frolova, Y. L. Dorokhov and J. G. Atabekov (2001)
Visualization by atomic force microscopy of Tobacco mosaic virus movement protein–RNA complexes formed in vitro
Journal of General Virology 82 (6), 1503-1508
Abstract: The structure of complexes formed in vitro by tobacco mosaic virus (TMV)-coded movement protein (MP) with TMV RNA and short (890 nt) synthetic RNA transcripts was visualized by atomic force microscopy on a mica surface. MP molecules were found to be distributed along the chain of RNA and the structure of MP–RNA complexes depended on the molar MP:RNA ratios at which the complexes were formed. A rise in the molar MP:TMV RNA ratio from 20:1 to 60–100:1 resulted in an increase in the density of the MP packaging on TMV RNA and structural conversion of complexes from RNase-sensitive 'beads-on-a-string' into a 'thick string' form that was partly resistant to RNase. The 'thick string'-type RNase-resistant complexes were also produced by short synthetic RNA transcripts at different MP:RNA ratios. The 'thick string' complexes are suggested to represent clusters of MP molecules cooperatively bound to discrete regions of TMV RNA and separated by protein-free RNA segments.
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Database assignments for author(s): Joseph G. Atabekov

Research topic(s) for pests/diseases/weeds:
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Tobacco mosaic virus