Journal of General Plant Pathology (2016) 82, 89-95
Chi-Chu Tsai, Chun-Yen Huang, I-Szu Weng, Wen-Lin Liu and Chang-Hung Chou (2016)
One-step RT-LAMP analysis for efficient detection of Odontoglossum ringspot virus from Oncidiinae hybrids (Orchidaceae)
Journal of General Plant Pathology 82 (2), 89-95
Abstract: Odontoglossum ringspot virus (ORSV) is one of the most serious pathogens in the orchid industry. The production of virus-free orchid seedlings is the most efficient strategy to counter this virus problem. Therefore, an easy, efficient process to detect viruses is important to the orchid industry. One-step reverse transcription (RT) loop-mediated isothermal amplification (LAMP) technique was developed to detect ORSV in Oncidiinae hybrids. RT-LAMP analysis primers were designed based on the conserved regions of the RNA-dependent RNA polymerase (RdRp) gene among ORSV isolates. RT-LAMP reaction conditions were optimized to detect ORSV from Oncidiinae hybrids. This one-step RT-LAMP method proved to be easy, efficient, sensitive, and inexpensive for detecting ORSV in orchids and is the first application of a one-step RT-LAMP to detect ORSV.
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Link to article at publishers website
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Odontoglossum ringspot virus | Oncidium (crop) | Taiwan |