Journal of General Plant Pathology (2015) 81, 173-179

Hiroshi Uematsu, Yasuhiro Inoue and Yasuo Ohto (2015)
Detection of Pantoea stewartii from sweet corn leaves by loop-mediated isothermal amplification (LAMP)
Journal of General Plant Pathology 81 (3), 173-179
Abstract: To improve the diagnosis of Stewart's wilt, we developed a loop-mediated isothermal amplification (LAMP) assay, based on two conserved sequences of the cpsD and pstS–glmS gene regions. The detection limit of the LAMP assay was 10⁴ colony-forming units/mL. No cross reaction was observed with other Pantoea spp., other genera associated with sweet corn diseases, or strains isolated from the surface of maize leaves in Japan. The LAMP reaction was not inhibited by leaf contents. The simplicity of sample preparation and short processing time make this LAMP assay useful for field surveillance of Stewart's wilt.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pantoea stewartii	Maize/corn (Zea mays)