Journal of General Plant Pathology (2010) 76, 122-131

Noriko Furuya, Keiichiro Matsukura, Kenta Tomimura, Mitsuru Okuda, Shin-Ichi Miyata and Toru Iwanami (2010)
Sequence homogeneity of the psi-serA-trmU-tufB-secE-nusG-rplKAJL-rpoB gene cluster and the flanking regions of `Candidatus Liberibacter asiaticus' isolates around Okinawa Main Island in Japan
Journal of General Plant Pathology 76 (2), 122-131
Abstract: In Japan and Southeast Asia, `Candidatus Liberibacter asiaticus' (Las) is the dominant causal agent of citrus greening (huanglongbing) disease. Using PCR techniques, we determined the 11168-nucleotide sequence of the psi-serA-trmU-tufB-secE-nusG-rplKAJL-rpoB gene cluster and the flanking regions for 51 Japanese, four Taiwanese, four Indonesian, and three Vietnamese isolates of Las. The sequence is identical in 62 isolates collected from Japan, Taiwan, Indonesia, and Vietnam, except for nucleotide substitutions at 11 positions. Some Las isolates from Sakishima Islands near Taiwan had unique nucleotide mutations, but all Las isolates around Okinawa Main Island were homologous. On the basis of the pattern of single nucleotide polymorphisms (SNPs) of the 11 nucleotide substitutions, the 62 Las isolates from Japan, Taiwan, Indonesia, and Vietnam could be divided into 12 pattern groups, and the 51 Japanese isolates consisted of six patterns. The results suggested that one unique genetic group is dominant around Okinawa Main Island, whereas several different are commonly distributed around islands near Taiwan.
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Link to article at publishers website
Database assignments for author(s): Toru Iwanami

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Liberibacter asiaticus	Citrus (genus)	Indonesia
	Liberibacter asiaticus	Citrus (genus)	Japan
	Liberibacter asiaticus	Citrus (genus)	Taiwan
	Liberibacter asiaticus	Citrus (genus)	Vietnam