Journal of General Plant Pathology (2010) 76, 1-6

Mari Narusaka, Tomonori Shiraishi, Masaki Iwabuchi and Yoshihiro Narusaka (2010)
Monitoring fungal viability and development in plants infected with Colletotrichum higginsianum by quantitative reverse transcription-polymerase chain reaction
Journal of General Plant Pathology 76 (1), 1-6
Abstract: Colletotrichum higginsianum causes typical anthracnose lesions on a wide range of cruciferous plants. Currently, assays for C. higginsianum infection of host plants rely on visual diagnosis of symptoms. In this study, we developed an assay to estimate the viability and development of C. higginsianum in Arabidopsis thaliana and Brassica rapa by detecting C. higginsianum-actin (Ch-ACT) mRNA using quantitative reverse transcription-PCR. Ch-ACT mRNA was specifically targeted because of its constitutive expression throughout the cell cycle. The methodology described will be useful for assessing potential antifungal compounds and breeding disease-resistant crops.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Tomonori Shiraishi

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Colletotrichum higginsianum