Journal of General Plant Pathology (2009) 75, 72-75

Hiroshi Kajihara, Kazuyuki Muramoto, Shin-Ichi Fuji, Shuhei Tanaka and Shin-ichi Ito (2009)
Simultaneous detection of Japanese yam mosaic virus and Yam mild mosaic virus from yam leaves using a tube capture reverse transcription-polymerase chain reaction assay
Journal of General Plant Pathology 75 (1), 72-75
Abstract: To detect Japanese yam mosaic virus (JYMV) and Yam mild mosaic virus (YMMV) in yam plants in Japan, we developed a duplex RT-PCR assay consisting of a tube-capture procedure followed by one-step RT-PCR with two primer pairs. A 241-bp fragment of the coat protein region of JYMV and a 174-bp fragment of the nuclear inclusion protein b region of YMMV were amplified, thus identifying the two viruses from yam plants cultivated in Yamaguchi Prefecture in 2007. All water yam plants examined were infected with YMMV alone. All the Japanese yam and Chinese yam plants were infected with either JYMV alone or both JYMV and YMMV, suggesting that YMMV and JYMV are prevalent among field-grown yam plants.
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Database assignments for author(s): Shin-ichi Ito, Shin-ichi Fuji

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Yam mild mosaic virus	Yam (Dioscorea)	Japan
	Japanese yam mosaic virus	Yam (Dioscorea)	Japan