Journal of General Plant Pathology (2008) 74, 434-437

Atsushi Ohkawa, Noriko Ishikawa-Suehiro, Seiichi Okuda and Tomohide Natsuaki (2008)
Construction of an infectious full-length cDNA clone of Chrysanthemum virus B
Journal of General Plant Pathology 74 (6), 434-437
Abstract: An infectious full-length cDNA clone of Chrysanthemum virus B (CVB, genus Carlavirus), was constructed. Four cDNA fragments covering the whole genome of CVB-S were cloned between the Cauliflower mosaic virus 35S promoter and the nopaline synthase (NOS) terminator. Chrysanthemum and garland chrysanthemum were inoculated with the constructed plasmid, named pCVB, using a gene gun system. As is the case in wild-type, CVB-infected plants, no visible symptoms were observed on plants inoculated with pCVB; however, western blotting and electron microscopy indicated the presence of the progeny virus of pCVB. pCVB could be a useful tool for analyzing the functions of carlaviral proteins.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Tomohide Natsuaki

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Chrysanthemum virus B