Journal of General Plant Pathology (2008) 74, 151-155

Naoya Urasaki, Shinji Kawano, Hiroyuki Mukai, Takashi Uemori, Osamu Takeda and Teruo Sano (2008)
Rapid and sensitive detection of `Candidatus Liberibacter asiaticus' by cycleave isothermal and chimeric primer-initiated amplification of nucleic acids
Journal of General Plant Pathology 74 (2), 151-155
Abstract: We developed a detection method for `Candidatus Liberibacter asiaticus', causal agent of citrus huanglongbing, using isothermal and chimeric primer-initiated amplification of nucleic acids combined with cycling probe technology (Cycleave ICAN). With Cycleave ICAN, the reaction was done in one tube in 1 h without the need for electrophoresis, and false positives were not generated. In addition, Cycleave ICAN method was more sensitive than the conventional PCR method. Cycleave ICAN helps shorten the time for the large-scale detection needed to manage huanglongbing.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Teruo Sano

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Liberibacter asiaticus	Citrus (genus)