Journal of General Plant Pathology (2003) 69, 194-198

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Hisashi Nishigawa, Kenro Oshima, Shin-ichi Miyata, Masashi Ugaki and Shigetou Namba (2003)
Complete set of extrachromosomal DNAs from three pathogenic lines of Onion yellows phytoplasma and use of PCR to differentiate each line
Journal of General Plant Pathology 69 (3), 194-198
Abstract: Two lines of onion yellows phytoplasma with reduced pathogenicity have been isolated from the original wild-type line (OY-W). One is a line with mild symptoms (OY-M) and the other is a non-insect-transmissible line, also with mild symptoms (OY-NIM). We previously reported heterogeneity in extrachromosomal DNA (EC-DNA) species in these lines. In this report, another EC-DNA, EcOYNIM, from OY-NIM was cloned and sequenced, providing a complete set of EC-DNAs from the three OY lines. To monitor each phytoplasma in synergism or cross-protection experiments, a pair of polymerase chain reaction (PCR) primers that universally amplify a portion of the EC-DNAs that are characteristic of each line was designed. Using this primer set, a line-specific fragment was amplified from the total DNA of each plant inoculated with one or more phytoplasma lines. The PCR product sizes differ for each phytoplasma line, so the lines can be distinguished even in plants infected with multiple lines. Because EC-DNAs are more abundant than chromosomal genes in phytoplasma cells, this primer set will be valuable for detecting and discriminating these phytoplasma lines and for analyzing their interaction.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Shigetou Namba

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
surveys/sampling/distribution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Phytoplasma asteris Japan