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Journal of General Plant Pathology (2002) 68, 326-332

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Fumiaki Usuki, Kazuhiko Narisawa, Miho Yonezawa, Makoto Kakishima and Teruyoshi Hashiba (2002)
An efficient inoculation method for colonization of Chinese cabbage seedlings by the root endophytic fungus Heteroconium chaetospira
Journal of General Plant Pathology 68 (4), 326-332
Abstract: To develop an efficient method to inoculate Chinese cabbage seedlings with the root endophytic fungus Heteroconium chaetospira, an appropriate nursery soil and glucose concentration for the nutrient medium for fungal colonization were determined. A grid-sheet method was established for estimating the degree of colonization of entire roots by the fungus. The fungus colonized at high frequencies when peat moss was used as the rooting medium. Colonization was highest (75%) when peat moss was amended with 0.1% glucose. Under these conditions, fungal hyphae developed intracellularly in root cortical cells. In contrast, under high glucose conditions, fungal colonization was restricted mostly to intercellular regions of epidermal or cortical root tissues. Here, hyphae formed inter- or intracellular microsclerotia.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Makoto Kakishima, Teruyoshi Hashiba, Kazuhiko Narisawa

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Heteroconium chaetospira (antagonist) Japan