Journal of Entomology (2007) 4, 167-180

Omar Hamarsheh, Wolfgang Presber, Ziad Abdeen, Samer Sawalha, Ahmad Al-Lahem and Gabriele Schonian (2007)
Sequence analysis of the ribosomal DNA ITS2 region for Phlebotomus papatasi (Diptera: Psychodidae)
Journal of Entomology 4 (3), 167-180
Abstract: We investigated the heterogeneity of the ribosomal internal transcribed spacer 2 (ITS2) within 131 individuals of Phlebotomus papatasi from 25 geographic localities in 10 countries by sequencing the PCR-amplified copies of this spacer. Sequence heterogeneity was observed in both sexes from all localities. There are many microsatellite sequences that show insertion-deletion polymorphisms. The length of the ITS2 ranged from 340 to 512 bp and the average GC content of the ITS2 was 29.2%. The phylogenetic relationships among the members of P. papatasi were inferred by neighbour-joining analysis. Although the relationships among P. papatasi populations could not be completely resolved, the phylogenies were in good agreement with previous enzyme electrophoresis studies.
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Database assignments for author(s): Omar Hamarsheh, Ziad A. Abdeen

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phlebotomus papatasi		Cyprus
	Phlebotomus papatasi		Italy
	Phlebotomus papatasi		Turkey
	Phlebotomus papatasi		Egypt
	Phlebotomus papatasi		Morocco
	Phlebotomus papatasi		Tunisia
	Phlebotomus papatasi		Israel
	Phlebotomus papatasi		Jordan
	Phlebotomus papatasi		Syria
	Phlebotomus papatasi		Palestinian Territories