Journal of Economic Entomology (2004) 97, 2079-2082

Vincent Le Roux, Julien Saguez, Charles Vincent and Philippe Giordanengo (2004)
Rapid method to screen resistance of potato plants against Myzus persicae (Homoptera: Aphididae) in the laboratory
Journal of Economic Entomology 97 (6), 2079-2082
Abstract: With the objective to develop a potato, Solanum tuberosum L., resistance program against aphids, we propose a rapid screening method with Myzus persicae (Sulzer) in the laboratory. We aimed to optimize the duration of the whole procedure and to decrease the frequency of measurements. In a first experiment, intrinsic rate of natural increase (r _m ) values were compared between adult aphids reared throughout their entire life and adults reared only during a period equivalent to their prereproductive period. No significant differences were observed. In a second experiment, four groups of aphids were distinguished according to the sampling frequency, i.e., those whose biological parameters were evaluated every single, second, third, and fourth day. Except for the fourth-day experiment, the r _m values estimated on aphids reared on the three potato lines were not significantly different whatever sampling frequency of single, second, or third day used to check aphids. Thus, screening efforts in laboratory can be largely optimized by evaluating adult aphids only during a period equivalent to their prereproductive period and assessing M. persicae populations every third day. Our method is reliable and adapted to screen a large number of potato plants against M. persicae because it allows an average 70% reduction in the time required for the whole experimental process.
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Database assignments for author(s): Charles Vincent, Julien Saguez, Philippe Giordanengo, Vincent Le Roux

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Myzus persicae	Potato (Solanum tuberosum)