Journal of Chemical Ecology (2009) 35, 542-551

Jeyasankar Alagarmalai, David Nestel, Daniela Dragushich, Ester Nemny-Lavy, Leonid Anshelevich, Anat Zada and Victoria Soroker (2009)
Identification of host attractants for the Ethiopian fruit fly, Dacus ciliatus Loew
Journal of Chemical Ecology 35 (5), 542-551
Abstract: The Ethiopian fruit fly, Dacus ciliatus, is an oligophagous pest of cucurbit crops, particularly melons, cucumbers, and marrows (summer squash). The present study aimed to identify host attractants for D. ciliatus and was guided by a behavioral bioassay and an electrophysiological assay. We tested volatile compounds from the fruits of a host plant, ripe and unripe Galia melon, Cucumis melo var. reticulates. Both sexes were attracted to melon volatiles. Those of ripe melon were preferred. Gas chromatography-electroantennographic detection analysis of the behaviorally active ripe melon volatiles consistently showed that 14 compounds elicited similar antennal responses from both sexes. Twelve compounds were identified by gas chromatography-mass spectrometry (GC-MS) using GC-MS libraries, retention indices (RI), and authentic standards. The electrophysiological activities of the compounds that were present at sufficient levels for identification, benzyl acetate, hexanyl acetate, (Z)-3-hexenyl acetate, (Z)-3-octenyl acetate, octanyl acetate, (Z)-3-decenyl acetate, and (E)-ß-farnesene, were evaluated at six different dosage levels by using electroantennography (EAG). Benzyl and hexanyl acetates elicited dose responses only in males, while other tested compounds elicited dose responses in both sexes. The strongest responses were observed for doses between 100 ng and 10 μg. The dose response, in terms of attractiveness to synthetic compounds within the active range (as determined by EAG), also was evaluated in the behavioral bioassay. Synthetic acetates were attractive to both sexes when tested individually. Significant attraction was observed when individual compounds were applied in the bioassay arena at doses of 0.5-1 μg/dispenser. Blends of compounds in equal proportions also were attractive to the insects. The most attractive blend was a mixture of four or five identified acetates. The addition of an equal proportion of (E)-ß-farnesene to this mixture had a deterrent effect.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Victoria Soroker, David Nestel

Research topic(s) for pests/diseases/weeds:
pheromones/attractants/traps
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Dacus ciliatus	Melon (Cucumis melo)