Journal of Chemical Ecology (2000) 26, 2401-2419

Qun Li, Sanford D. Eigenbrode, G.R. Stringam and M.R. Thiagarajah (2000)
Feeding and growth of Plutella xylostella and Spodoptera eridania on Brassica juncea with varying glucosinolate concentrations and myrosinase activities
Journal of Chemical Ecology 26 (10), 2401-2419
Abstract: We measured feeding behavior, feeding damage, and larval growth of the crucifer specialist, Plutella xylostella and the generalist, Spodoptera eridania, on the cotyledons of 14 homozygous lines of Brassica juncea differing in myrosinase activity and glucosinolate profiles. The proportion of time feeding and area damaged by P. xylostella were lower on lines with high myrosinase activities [0.49-0.73 nmol glucose released/mg tissue(fresh weight, FW)/min] than on lines with low myrosinase activities [0.20-0.31 nmol glucose released/mg tissue(FW)/min]. In contrast, the proportion of time feeding and area damaged by S. eridania were not related to myrosinase activity, but were lower on cotyledons of lines with high glucosinolate concentrations [6.8-21.3 µg/g(FW)] than on lines with low glucosinolate concentrations [0.09-0.61 µg/g(FW)]. Relative growth rates (RGR) of both insect species were lower on lines with high glucosinolate concentrations, but were not related to myrosinase activity in the lines. In toxicity experiments that used artificial diets, allyl isothiocyanate, but not allyl glucosinolate, was lethally toxic to neonate P. xylostella (LC₅₀s of 1.54 µmol/g. and > 100 µmol/g, respectively), whereas isothiocyanate and the glucosinolate were lethally toxic to neonate S. eridania (LC₅₀s of 3.42 and 6.73 µmol/g, respectively). We interpret these results to indicate that myrosinase activity might be more important for plant defense against specialist insects that have adaptations to intact glucosinolates, but less important for defense against generalists, which are susceptible to the intact glucosinolates.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Sanford D. Eigenbrode

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella	Brassica - other species
	Spodoptera eridania	Brassica - other species