Journal of Applied Entomology (2019) 143, 1072-1079

Thiago Mastrangelo, Fabiano França da Silva, Gabriel Moura Mascarin and Clíssia Barboza da Silva (2019)
Multispectral imaging for quality control of laboratory-reared Anastrepha fraterculus (Diptera: Tephritidae) pupae
Journal of Applied Entomology 143 (10), 1072-1079
Abstract: The sterile insect technique (SIT) has been widely used to suppress several fruit fly species. In southern Brazil, millions of sterile flies of the South American fruit fly, Anastrepha fraterculus Wiedemann (Dipetra: Tephritidae), will be produced in a mass-rearing facility called MOSCASUL to suppress wild populations from commercial apple orchards. In spite of standard rearing conditions, the quality of pupal batches can be inconsistent due to various factors. The quantification of poor quality material (e.g. empty pupae, dead pupae or larvae) is necessary to track down rearing issues, and pupal samples must be taken randomly and evaluated individually. To speed up the inspection of pupal samples by replacing the manual testing with the mechanized one, this study assessed a multispectral imaging (MSI) system to distinguish the variations in quality of A. fraterculus pupae and to quantify the variations based on reflectance patterns. Image acquisition and analyses were performed by the VideometerLab4 system on 7-d-old pupae by using 19 wavelengths ranging from 375 to 970 nm. The image representing the near infrared wavelength of 880 nm clearly distinguished among high-quality pupae and the other four classes (i.e. low-quality pupae, empty pupae, dead pupae and larvae). The blind validation test indicated that the MSI system can classify the fruit fly pupae with high accuracy. Therefore, MSI-based classification of A. fraterculus pupae can be used for future pupal quality assessments of fruit flies in mass-rearing facilities.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Thiago Mastrangelo

Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anastrepha fraterculus	Apple (Malus)	Brazil (south)