Journal of Applied Entomology (2003) 127, 167-173

G. Smagghe, B.P. Braeckman, N. Huys and H. Raes (2003)
Cultured mosquito cells Aedes albopictus C6/36 (Dip., Culicidae) responsive to 20-hydroxyecdysone and non-steroidal ecdysone agonist
Journal of Applied Entomology 127 (3), 167-173
Abstract: A cell line derived from an important mosquito, Aedes albopictus Skuse, was responsive to treatments with the insect moulting hormone 20-hydroxyecdysone (20E) and two new insecticides, RH-5849 and RH-5992, by cell clumping and inhibition of cell proliferation. The Aedes C6/36 cells showed typical numerous long extensions, and fluorescent BODIPY^®-phalloidin staining demonstrated that they resulted from F-actin polymerization. In addition, the ultrastructure of treated cells showed a high degree of vacuolarization that is in accord with protein synthesis. SDS-PAGE demonstrated that three proteins were induced that may belong to the hsp70 family. In conclusion, an insect cell line that responds to 20E may be useful for screening and identifying hormone analogues and novel insecticides.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Guy Smagghe

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aedes albopictus