International Journal of Tropical Insect Science (2021) 41, 1285-1292

Nuria Morfin, Hanan A. Gashout, José O. Macías-Macías, Alvaro De la Mora, José C. Tapia-Rivera, José M. Tapia-González, Francisca Contreras-Escareño and Ernesto Guzman-Novoa (2021)
Detection, replication and quantification of deformed wing virus-A, deformed wing virus-B, and black queen cell virus in the endemic stingless bee, Melipona colimana, from Jalisco, Mexico
International Journal of Tropical Insect Science 41 (2), 1285-1292
Abstract: Melipona colimana is a stingless bee endemic to the temperate regions of Jalisco, Mexico, where it provides pollinating services and is cultured for the production of honey and cerumen. There is little information about the sanitary status of M. colimana, but it is critical to know more about it to keep these bees healthy and productive. The proximity of M. colimana colonies to commercial honey bee (Apis mellifera) colonies could facilitate pathogens transmission between the two species. We determined the presence, replication, and levels of two variants of deformed wing virus (DWV-A and DWV-B), and black queen cell virus (BQCV) in samples of M. colimana and A. mellifera. We also compared the DWV-A variant with variants from other regions of the world. Both A. mellifera and M. colimana were positive to DWV-A and BQCV, but negative to DWV-B. Quantification analyses showed that A. mellifera had significantly higher levels of DWV-A and BQCV than did M. colimana. Additionally, evidence of DWV-A and BQCV replication in M. colimana was demonstrated by visualizing the minus-strand of DWV and BQCV RNA using tagged primers for RT-PCR. The DWV-A variant did not seem to be atypical compared to variants from other world regions. Due to the importance of M. colimana for the conservation of regional ecosystems as well as for its use for honey and cerumen production, the potential impact of multiplying honey bee viruses in M. colimana populations deserves further investigation.
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Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Triatovirus nigereginacellulae		Mexico
	Iflavirus aladeformis		Mexico