Insect Science (2005) 12, 225-229

Shao-Li Wang, Cheng-Fa Sheng, Chuan-Ling Qiao and Miyata Tadashi (2005)
cDNA sequence analysis of ribosomal protein S13 gene in Plutella xylostella (Lepidoptera: Plutellidae)
Insect Science 12 (3), 225-229
Abstract: Ribosomal protein S13 gene has been cloned and analyzed in many organisms, but there are few documents relating to insects. In this communication, the full-length cDNA sequence of ribosomal protein S13 gene in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae), was determined by using PCR amplification technique. The features of the ribosomal protein S13 gene sequence were analyzed and the deduced amino acids sequence was compared with those from other insects. The results of multi-alignment of the amino acid sequences between the diamondback moth and other insect species revealed that this gene sequence is highly conserved in insects. Based on maximum likelihood method, a phylogenetic tree was constructed from 10 different species using PHYLIP software. It showed that nematode is one separate lineage and the five insect species belong to another lineage, whereas those species higher than insects form the third one. The pattern of this phylogenetic tree evidently represented the evolution of different species.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Shaoli Wang

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella