Formosan Entomologist (2003) 23, 225-237

Yu-Cheng Su, Err-Lieh Hsu and Chau-Mei Ho (2003)
[Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to distinguish variations of Aedes aegypti based on abdominal tergal scales]
Formosan Entomologist 23 (3), 225-237
Abstract: There is much variation in the distribution of white scaling on the abdominal tergites of Aedes aegypti. The C.K. Meek's (CKM) classification system was used to identify and rank eight classes in the abdominal scale patterns, but only seven classes were found in Taiwan, including CKM1. which was generalized as the type form of Aedes aegypti and CKM2 to CKM7 which were concluded to be the pale form. Pure CKM strains were selected by single-pair matings, and genomic DNA of these mosquitoes was extracted in order to detect the variations in RAPD patterns. The RAPD fragments profiles of seven strains with different scale pattern were analyzed and genetic similarities were estimated between each two strains using two different statistic methods. The UPGMA dedrogram method illustrated the relatedness of these strains, and both statistical methods assorted these strains into two major groups: the type form and the pale form. But the genetic distance between each pair of strains differed using these two methods. These results indicate that the RAPD-PCR technique is sufficiently sensitive for studies of mosquito molecular taxonomy, especially at intra-species level.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Err-Lieh Hsu

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aedes aegypti		Taiwan