Fitopatologia Brasileira (2004) 29, 651-655

Hudson Teixeira, Maria das Graças G.C. Vieira and José C. Machado (2004)
Marcadores RAPD na análise da diversidade genética de isolados de Acremonium strictum
[RAPD markers in the genetic diversity analysis of Acremonium strictum isolates]
Fitopatologia Brasileira 29 (6), 651-655
Abstract: Differentiation between Acremonium strictum and Fusarium verticillioides infecting corn (Zea mays) seeds is difficult because they present some morphological similarities. Molecular techniques have been applied with success, allowing the development of fast, sensible and specific diagnostic methods for plant pathogens in complement to morphological analysis. The objectives of this work were to characterize and measure the genetic diversity of ten isolates of A. strictum associated with maize (Zea mays) seeds from different brazilian regions, using DNA analysis (RAPD) in comparison with one isolate of F. verticillioides. Twenty-five Operon primers generated polymorphisms that were efficient in grouping A. strictum isolates and in distinguishing them from F. verticillioides. The isolates of A. strictum showed variable intraspecific diversity between 3.4% to 44.4%. No genotypic symilarities were correlated with the geographic origin of the A. strictum isolates.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sarocladium strictum	Maize/corn (Zea mays)	Brazil (south)