Fitopatologia Brasileira (2002) 27, 635-638

Hugo Kuniyuki, Jorge A.M. Rezende, Valdir A. Yuki and Juarez A. Betti (2002)
Identificação sorológica do vírus do mosaico das nervuras da videira no Brasil
[Serological identification of the Grapevine fleck virus in Brazil]
Fitopatologia Brasileira 27 (6), 635-638
Abstract: In the State of São Paulo, four different isolates of the virus that induces chlorotic translucent vein break in the minor veins of grapevines (Vitis spp.) were separated by their differential biological behaviour and tested for their relationship to Grapevine fleck virus (GFkV) by DAS-ELISA and TAS-ELISA with commercial antisera against GFkV. Young leaves from spring shoots and dormant cuttings were used for antigen extraction. All infected plants were previously indexed by graft-inoculation to 'Rupestris du Lot'. The four isolates gave positive reactions to the GFkV antiserum. Positive ELISA reactions were observed in 66 samples of 26 varieties, from 11 grape-growing areas from the State of São Paulo as well as 24 samples, of 12 varieties, from five other states of Brazil. Healthy plants consistently gave negative results. Young leaves were better sources of antigen than cortical scrapings of dormant cuttings. The results showed that the four isolates have a serological relationship with the GFkV, suggesting that they belong to the viral complex that induce grapevine fleck disease.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Database assignments for author(s): Jorge A.M. Rezende

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Maculavirus vitis	Grapevine (Vitis)	Brazil (south)