Fitopatologia Brasileira (2002) 27, 58-64

Thor V.M. Fajardo, Gilmar B. Kuhn, Marcelo Eiras and Osmar Nickel (2002)
Detecção de Closterovirus em videira e caracterização parcial de um isolado do Grapevine leafroll-associated virus 3
[Detection of Closterovirus in grapes and partial characterization of an isolate of Grapevine leafroll-associated virus 3]
Fitopatologia Brasileira 27 (1), 58-64
Abstract: Grapevine (Vitis spp.) leafroll is a disease caused by up to eight viruses, denominated Grapevine leafroll-associated viruses (GLRaV- 1 to -8), which are serologically unrelated and associated with phloem tissue. In the present paper, GLRaV-1 and -3 were detected by DAS-ELISA in 6.9 and 14.7% of the samples, respectively, and GLRaV-2, -5 and -7 were not detected in grapes. The analysed samples came from two of the most important grape growing regions in Brazil (Serra Gaúcha and Vale do São Francisco). The GLRaV-3 was also identified by dot-ELISA and western blot, associated with a probable coat protein subunit of approximately 36 kDa. A fragment of 340 bp, comprising the 3' terminal of the viral polymerase gene, was PCR-amplified and sequenced. The nucleotide and deduced amino acid sequences of this isolate showed high homology, 95 and 97.1%, respectively, with another GLRaV-3 isolate (NY1).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Database assignments for author(s): Marcelo Eiras

Research topic(s) for pests/diseases/weeds:
damage/losses/economics

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Grapevine leafroll-associated virus 3	Grapevine (Vitis)	Brazil (south)