FEMS Microbiology Letters (2007) 270, 42-48
Mark R.H. Hurst, Sam S. Beard, Trevor A. Jackson and Sandra M. Jones (2007)
Isolation and characterization of the Serratia entomophila antifeeding prophage
FEMS Microbiology Letters 270 (1), 42-48
Abstract: The Serratia entomophila antifeeding prophage (Afp) is thought to form a virus-like structure that has activity towards the New Zealand grass grub, Costelytra zealandica. Through the trans based expression of AnfA1, an RfaH - like transcriptional antiterminator, the Afp, was able to be induced. The expressed Afp was purified and visualized by electron microscopy. The Afp resembled a phage tail-like bacteriocin, exhibiting two distinct morphologies: an extended and a contracted form. The purified Afp conferred rapid activity towards C. zealandica larvae, causing cessation of feeding and a change to an amber colouration within 48 h postinoculation, with increased dose rates causing larval mortality.
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Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Costelytra giveni | ||||
Serratia entomophila (entomopathogen) | Costelytra giveni |