FEMS Microbiology Letters (2005) 251, 81-89

Eric Vivien, Sandrine Megessier, Isabelle Pieretti, Stéphane Cociancich, Roger Frutos, Dean W. Gabriel, Philippe C. Rott and Monique Royer (2005)
Xanthomonas albilineans HtpG is required for biosynthesis of the antibiotic and phytotoxin albicidin
FEMS Microbiology Letters 251 (1), 81-89
Abstract: Xanthomonas albilineans, the causal agent of leaf scald disease of sugarcane, produces a highly potent polyketide-peptide antibiotic and phytotoxin called albicidin. Previous studies established the involvement of a large cluster of genes in the biosynthesis of this toxin. We report here the sub-cloning and sequencing of an additional gene outside of the main cluster and essential for albicidin biosynthesis. This gene encodes a 634-amino-acid protein that shows high identity with the Escherichia coli heat shock protein HtpG. Complementation studies of X. albilineans Tox^- mutants confirmed the requirement of htpG for albicidin biosynthesis and revealed functional interchangeability between E. coli and X. albilineans htpG genes. HtpG was co-localised with albicidin in the cellular membrane, i.e., the cellular fraction where the toxin is most probably biosynthesised. Here we show the requirement of an HtpG protein for the biosynthesis of a polyketide-peptide antibiotic.
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Link to article at publishers website
Database assignments for author(s): Dean W. Gabriel, Monique Royer

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas albilineans