FEMS Microbiology Letters (2005) 247, 101-104

Lobna Abdelkefi Mesrati, Slim Tounsi, Fakher Kamoun and Samir Jaoua (2005)
Identification of a promoter for the vegetative insecticidal protein-encoding gene vip3LB from Bacillus thuringiensis
FEMS Microbiology Letters 247 (1), 101-104
Abstract: The expression of vip3LB, one of the vegetative insecticidal protein-encoding genes of Bacillus thuringiensis, was studied at the transcriptional level. By primer extension analysis, we have identified, for the first time, the transcription start point of vip3-type gene. Upstream from vip3LB transcription start point, was found a nucleotide sequence partially homologous to the consensus sequence for the E sigma^E holoenzyme of B. subtilis. Thus, it was strongly suggested that the identified vip3 promoter was under the control of sigma³⁵-like enzyme, the B. thuringiensis homolog of sigma^E. The transcriptional activity from the promoter was detected at the vegetative stage of growth starting at mid-log phase as well as during the middle stage of sporulation.
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Link to article at publishers website
Database assignments for author(s): Samir Jaoua, Slim Tounsi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Bacillus thuringiensis Vip-toxins (entomopathogen)