Experimental and Applied Acarology (2000) 24, 645-659
G. Das, S. Ghosh, M.H. Khan and J.K. Sharma (2000)
Immunization of cross-bred cattle against Hyalomma anatolicum anatolicum by purified antigens
Experimental and Applied Acarology 24 (8), 645-659
Abstract: Extracts prepared from unfed larvae of Hyalomma anatolicum anatolicum were purified by immunoaffinity chromatography using anti-gut IgG as ligand. Affinity purified antigen (Aff-GHLAg) was used to immunize cross-bred (Bos taurus × B. indicus) calves of 6-7 months of age. Immunized calves rejected 70.6% larvae, 54.5% nymphs and 61.9% adults. No significant changes in the engorged weight of females was observed; however, significant decrease in the engorgement weight of larvae and nymphs was recorded. There was a significant decrease in the emerging nymphs (p < 0.05) and adults (p < 0.01) of the tick stages fed on immunized animals. SDS-PAGE analysis revealed three antigenic proteins of 100, 59.4 and 37 kDa responsible for induction of resistance in the host.
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Database assignments for author(s): Srikant Ghosh
Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
control - general
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Hyalomma anatolicum |