European Journal of Plant Pathology (2022) 163, 167-179

Jan van der Wolf, Marjon Krijger, Odette Mendes, Balazs Brankovics, Peter Bonants, Leonie Didden and Ellis Meekes (2022)
Molecular characterization of Xanthomonas species isolated from Araceae and the development of a triplex TaqMan assay for detection of Xanthomonas phaseoli pv. dieffenbachiae
European Journal of Plant Pathology 163 (1), 167-179
Abstract: In total 58 Xanthomonas strains isolated from Araceae worldwide, together with 13 other phylogenetically-related Xanthomonas strains, were characterized using multilocus sequence analysis based on concatenated sequences of seven single copy orthologous genes, extracted from whole genome sequences. The analysis revealed a monophyletic clade of 48 strains, 44 isolated from Anthurium, identified as X. phaseoli pv. dieffenbachiae (Xpd) confirmed by nucleotide identity analysis. The other strains from aroids were identified as Xanthomonas euvesicatoria (2 strains), X citri (5 strains) and Xanthomonas sacchari (3 strains). Two TaqMan assays were designed for specific detection of Xpd, one targeting sequences of a hypothetical protein and one targeting a type I restriction endonuclease subunit S. The two assays showed similar reaction kinetics and were merged with an assay comprising an amplification and extraction control into a triplex assay. The assay was able to detect minimally 100 copies of a target sequence delivered as a gBlock, 100 fg of genomic DNA and 10⁴ cells per mL in an Anthurium leaf extract.
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Database assignments for author(s): Jan M. van der Wolf, Peter J. M. Bonants

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas phaseoli pv. dieffenbachiae	Anthurium (crop)	Netherlands
	Xanthomonas phaseoli pv. dieffenbachiae	Anthurium (crop)	U.S.A. (Hawaii)